CASNumber9001-67-6
ECNumber232-624-6
EnzymeCommission(EC)Number3.2.1.18 ( BRENDA
| IUBMB )MDLnumberMFCD00131711
Popular Documents: Datasheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.2.x.xGlycosidases,3.x.x.xHydrolases,ApplicationIndex,BiochemicalsandReagents,CarbohydrateHydrolysis, |
| QualityLevel | PREMIUM |
| type | TypeIII |
| form | bufferedaqueoussolution |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| foreignactivity | ProteaseandNAN-aldolase,present |
| storagetemp. | 2-8°C |
Description:
Application
Neurminidaseisusedasacell-surfaceprobeforglycoconjugatedistributionandinsubstratespecificitystudies.
NeuraminidasefromVibriocholeraehasbeenusedinastudytoassessitsroleinthebindinganduptakeofcholeratoxinbysusceptIBLecells.Ithasalsobeenusedinastudytoinvestigatethepreparationofasodiumsubstrateanditsuseinafluorometricassayofneuraminidase.
Generaldescription
Neuraminidaseenzymesareglycosidehydrolaseenzymesthatcatalyzehydrolysisofterminalsialicacidresidues.Themostwell-knownaretheviralnearamidases,whichpromoteinfluenzavirusrelease.
Packaging
1unitinpolybottle
2unitsinpolybottle
PreparationNote
Chromatographicallypurified.
Quality
Preservativefree.
UnitDefinition
Oneunitwillliberate1.0μmoleofN-acetylneuraminicacidperminatpH5.0at37°CusingNAN-lactoseorbovinesuBMAxillarymucin,unlessotherwisespecified.PricesbasedonunitsusingNAN-lactoseassubstrate.
Physicalform
Aqueoussolution,pH5.5,containing0.15MNaCland4mMCaCl2.
Biochem/physiolActions
Vibriocholeraeneuraminidasehasbeenshowntopromotecholeratoxininfectionbybindingthetoxinandaidinginitsuptakebycells.
