CASNumber9001-67-6
ECNumber232-624-6
EnzymeCommission(EC)Number3.2.1.18 ( BRENDA
| IUBMB )MDLnumberMFCD00131711
Popular Documents: SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.2.x.xGlycosidases,3.x.x.xHydrolases,ApplicationIndex,BiochemicalsandReagents,CarbohydrateHydrolysis, |
| QualityLevel | ELITE |
| type | TypeV |
| form | lyophilizedpowder |
| activity | ≥0.1 units/mgsolid(usingmucin) |
| ≥1.3 units/mgsolid(using4MU-NANA) | |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| foreignactivity | ProteaseandNAN-aldolase,present |
Description:
AnalysisNote
Packagesizesbasedon4MU-NANAunits
Packagesizesbasedonthe4MU-NANAunits
Generaldescription
Neuraminidaseenzymesarehydrolaseenzymesthatpromoteinfluenzavirusreleasefrominfectedcellsandfacilitatevirusspread.
Packaging
2.5,6unitsinglassbottle
25,250unitsinpolybottle
PreparationNote
Preparedbysaltfractionation.
Application
NeuraminidasefromClostridiumperfringenshasbeenusedinastudytoassessbindingwithhumanTlymphocytesinsheeppretreatedwithneuraminidase.Ithasalsobeenusedinastudytoinvestigatetheeffectofbilesaltsontheactionofhydrolysisbyneuraminidase.
Biochem/physiolActions
Neuraminidasecanincreaseaggregationincertaincelllinesbyremovingexposednegativelychargedsialicacidresiduesonthecellsurface.
Neuraminidasecleavageofsialicacidgroupshasbeenusedtostudyrecognitionbyantibodiesofglycoproteinstructures.TheuseofneuraminidaseintheestimationofN-acetylneuraminicacidwascomparedfavorablytotwoothermethods.
NeuraminidasesareusedtocleaveterminalN-acetylneuraminicacid(sialicacid)fromavarietyofglycoproteins.TheenzymefromClostridiumperfringenscleavesterminalsialicacidresidueswhichareα-2,3-α-2,6-orα-2,8-linkedtoGal,GlcNac,GalNAc,AcNeu,GlcNeu,oligosaccharides,glycolipidsorglycoproteins.Therelativerateofcleavagedecreasesintheorder:α-2-3>α-2-6.α-2-8.NeuraminidasefromC.perfringenscleavesα-2-3linkedsialicacidresiduesmostefficiently,comparedtoA.ureafaciens,(SigmaN3642)whichpreferentiallycleavesα-2-6linkedresidues.
Theuseofneuraminidasetoremovesialicacidresiduesfromglycoproteinsoncellsurfaceshasbeenfrequentlyreported.Generally,procedureshaveindicatedusingneuraminidaseinPBSat37°Cfor30minutes,followedbyseveralwashingswithPBS.Treatmentoftissuesectionswithneuraminidaseatmuchlowerconcentrationsrequirelongerincubation:for1-4U/mLin0.1MacetatebufferpH4.2-5,from2to20hoursat37°C.
Protocols&Applications
SialicAcidSynthesisandSignalingOverview
