• CASNumber9024-13-9

•  ECNumber232-777-9

•  EnzymeCommission(EC)Number4.2.2.4  ( BRENDA | IUBMB )

•  MDLnumberMFCD00130795

• Popular Documents:  Datasheet(PDF)  |  SpecificationSheet(PDF) 

Properties:

Description:

FrequentlyAskedQuestions

FrequentlyAskedQuestionsareavailableforthisProduct.

OtherNotes

Viewmoreinformationonenzymesforcomplexcarbohydrateanalysisatwww.Sigma-Aldrich.com/enzymeexplorer

Packaging

2,5,10unitsinglassbottle

PackagesbasedonchondroitinaseCactivity.

PreparationNote

Reconstituteina0.01%bovineserumalbuminaqueous(BSA)solution.Subsequentdilutionscanbemadeintoabuffercontaining50mMTris,pH8.0,with60mMsodiumacetateand0.02%BSA.Solutionsshouldbefreshlyprepared.

UnitDefinition

Oneunitwillliberate1.0μmoleof2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronicacid)-4-O-sulfo-D-galactosefromchondroitinsulfateAor1.0μmoleof2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronicacid)-6-O-sulfo-D-galactosefromchondroitinsulfateCperminatpH8.0at37°C.

Physicalform

LyophilizedpowdercontainingTrisbuffersalts

Application

ChondroitinaseABCfromSigmahasbeenusedtodigestO-linkedchondroitinsulfate-likeglycosaminoglycan(CSGAG).Theenzymehasalsobeenusedtodigestglycosaminoglycanchainsinproteoglycansduringtheconstructionandexpressionofarecombinantversicangeneinmammaliansystems.FurThermore,ithasbeenusedtodigestchondroitinsulfateintheproteoglycancomplexes.Theenzymehasalsobeenusedintheremovalofglycanchainsfromaggrecan,whicheliminatestheaxongrowth-inhibitoryeffectofaggrecan.

Biochem/physiolActions

ChondroitinaseABCcatalyzestheeliminativedegradationofpolysaccharidescontaining(1-4)-β-D-hexosaminyland(1-3)-β-D-glucuronosyl(or(1-3)-α-L-Iduronosyl)linkagestodisaccharidescontaining4-deoxy-β-D-gluc-4-enuronosylgroups.Itactsonchondroitin4-sulfate,chondroitin6-sulfate,anddermatansulfate.However,itactsslowlyonhyaluronate.Themolecularweightisapproximately120kDawith2non-identicalsubunitsofmolecularmasses86kDaand32kDa.ThepHoptimumis8.0withchondroitinsulfateand6.8withhyaluronicacid.Theoptimaltemperatureis37°C.1mMZn²⁺actsasaninhibitor,while0.05Macetateisanactivatoroftheenzyme.

Safety: