• CASNumber9024-13-9

•  ECNumber232-777-9

•  EnzymeCommission(EC)Number4.2.2.4  ( BRENDA | IUBMB )

•  MDLnumberMFCD00130795

• Popular Documents:  Datasheet(PDF)  |  SpecificationSheet(PDF) 

Properties:

Description:

OtherNotes

ContainspotassiumphosphatebuffersaltsandstABIlizer

Viewmoreinformationonenzymesforcomplexcarbohydrateanalysisatwww.Sigma-Aldrich.com/enzymeexplorer

Packaging

5,10unitsinglassbottle

Packagesbasedonchondroitinase Cactivity.

PreparationNote

Affinitypurified

UnitDefinition

Oneunitwillliberate1.0μmoleof2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronicacid)-4-O-sulfo-D-galactosefromchondroitinsulfateAor1.0μmoleof2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronicacid)-6-O-sulfo-D-galactosefromchondroitinsulfateCperminatpH8.0at37°C.

Application

Theenzymehasbeenusedtostudytheconsequencesofinducingacute,long-lastingchangesinchondroitinsulfateproteoglycansusingadultratbraincells.GlycosaminoglycanshavebeendigestedwithchondroitinaseABCforchondroitin/dermatansulfatequantitationinkeratocytecellculturesobtainedfromfreshbovinecornealstromae.

Biochem/physiolActions

ChondroitinaseABCcatalyzestheeliminativedegradationofpolysaccharidescontaining(1-4)-β-D-hexosaminyland(1-3)-β-D-glucuronosylor(1-3)-α-L-Iduronosyllinkagestodisaccharidescontaining4-deoxy-β-D-gluc-4-enuronosylgroups.Itactsonchondroitin4-sulfate,chondroitin6-sulfate,anddermatansulfate,andactsslowlyonhyaluronate.Themolecularweightisfoundtobeapproximately120kDawith2non-identicalsubunitsofmolecularmasses86kDaand32kDa.ThepHoptimumisfoundtobe8.0withchondroitinsulfateand6.8withhyaluronicacidandtemperatureoptimumis37°C.1mMZn²⁺actsasaninhibitor,while0.05Macetateisanactivatoroftheenzyme.

Safety: