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Sigma-Aldrich/Butyrylcholinesterase from equine serum/C1057-1KU/1KU
CASNumber9001-08-5
ECNumber232-579-2
EnzymeCommission(EC)Number3.1.1.8 ( BRENDA
| IUBMB )MDLnumberMFCD00081455
Popular Documents: Datasheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.1.x.xActingonesters,3.x.x.xHydrolases,AnalyticalandIndustrialEnzymes,ApplicationIndex,BiochemicalsandReagents, |
| QualityLevel | PREMIUM |
| form | lyophilizedpowder |
| composition | Protein,≥10% |
| solubility | H2O:soluble10 mg/mL |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| storagetemp. | −20°C |
Description:
AnalysisNote
Proteindeterminedbybiuret
Packaging
1000,5000unitsinglassbottle
UnitDefinition
Oneunitwillhydrolyze1.0 μmoleofbutyrylcholinetocholineandbutyrateperminatpH 8.0at37 °C.Theactivityobtainedusingbutyrylcholineassubstrateis~2.5timesthatobtainedusingacetylcholine.
Physicalform
Highlypurified;containsbuffersalts
Application
SelectiveinhibitionofBChEactivitycanbeusedinthedetectionoforganophosphates.Itsuseinthetreatmentoforganophosphatetoxicityhasbeenexplored.IthasbeenreportedthatthelevelofBChEinhumanbloodcorrelatestothedegreeofprotectionagainstpotentiallytoxicnerveagents.CholinesteraseshavealsobeeninvestigatedfortheirroleinAlzheimer’sdisease.
Biochem/physiolActions
Butyrylcholinesterase(BChE)isaserinehydrolasethatisstructurallysimilartoacetylcholinesterase(AChE),butdiffersinsubstratespecificitiesandinhibitorsensitivities.BChEcan,unlikeAChE,efficientlyhydrolyzelargerestersofcholinesuchasbutyrylcholineandbenzoylcholine.Theenzymeisatetramericglycoproteinwithfourequalsubunits(110kDaeach).TheenzymeisactivatedbyCa2+andMg2+.TheactivityisconstantoverthepHrangeof6.0-8.0.Itisinhibitedbybetaine,nicotine,organophosphates,andcarbamate.
