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Sigma-Aldrich/Carbonic Anhydrase from bovine erythrocytes/C3934-100MG/100MG
CASNumber9001-03-0
ECNumber232-576-6
EnzymeCommission(EC)Number4.2.1.1 ( BRENDA
| IUBMB )MDLnumberMFCD00081467
Popular Documents: SpecificationSheet(PDF)
Properties:
| RelatedCategories | 4.2.x.xC-Obonds,4.x.x.xLyases,AnalyticalandIndustrialEnzymes,ApplicationIndex,BiochemicalsandReagents, |
| form | lyophilizedpowder |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| storagetemp. | 2-8°C |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestionsareavailableforthisProduct.
Packaging
100,500mginpolybottle
UnitDefinition
OneWilbur-Anderson(W-A)unitwillcausethepHofa0.02MTrizmabuffertodropfrom8.3to6.3perminat0°C.(OneW-AunitisessentiallyequivalenttooneRoughton-Boothunit.)
Application
Carbonicanhydrasefrombovineerythrocytes(BCA)hasbeenusedtostudytheeffectofremovalofenzyme-boundmetalion,Zn2+,onaggregationbehavioroftheenzyme.RemovalofmetalionbyachelatorsuchasEDTAenhancesthepropensityoftheenzymetoadoptthemolten-globulestate.Thisstateoftheenzymeisfoundtobindtothechaperone-likeα-crystallinandpreventaggregation.TheenzymefromSigmahasbeenimmobilizedtoelectRochemicaltransducersinordertoobtaindevelopananalyticaldevicefordissolvedCO2measurement.IthasalsobeenusedfortheThermodynamicanalysisofconformationalchangesinBCA.
Biochem/physiolActions
Carbonicanhydraseisazincmetalloenzymethathasamolecularweightofapproximately30,000Da.Theenzymecatalyzesthehydrationofcarbondioxidetocarbonicacid.ItisinvolvedinvitalphysiologicalandpathologicalprocessessuchaspHandCO2homeostasis,transportofbicarbonateandCO2,biosyntheticreactions,boneresorption,calcification,andtumOrigenicity.Therefore,thisenzymeisanimportanttargetforinhibitorswithclinicalapplicationsforvariouspathologiessuchasglaucoma,epilepsyandParkinson’sdisease.
