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Linkage

Inadditiontokits,theindividualreagentsandglycerolstandardareavailableseparatelywhenfewerreactionsareneeded.

Packaging

Thekitalsoincludessufficientreagentforanadditional250freeglyceridetestsfortruetriglyceridedetermination.

Application

ASerumTriglycerideDeterminationKitwasusedtodeterminetheleveloftriglyceridesinwhole-bodyhomogenatesofDrosophilamelanogaster.

TheSerumTriglycerideDeterminationKitcanbeusedforthemeasurementofglycerol,truetriglycerides,ortotaltriglyceridesinserumorplasma.Theprocedureinvolvesenzymatichydrolysisbylipaseofthetriglyceridestoglycerolandfreefattyacids.Theglycerolproducedisthenmeasuredbycoupledenzymereactions.Manyofthetriglyceridereagentswhicharecommerciallyavailable,donotdifferentiatebetweenendogenousglycerolandglycerolderivedbyhydrolyticactionoflipaseonglycerides.
Triglyceridesarefirsthydrolyzedbylipoproteinlipasetoglycerolandfreefattyacids.Glycerolisthenphosphorylatedbyadenosine-5′-triphosphate(ATP)formingglycerol-1-phosphate(G-1-P)andadenosine-5′-diphosphate(ADP)inthereactioncatalyzedbyglycerolkinase(GK).G-1-Pisthenoxidizedbyglycerolphosphateoxidase(GPO)todihydroxy-acetonephosphate(DAP)andhydrogenperoxide(H₂O₂).Peroxidase(POD)catalyzesthecouplingofH₂O₂with4-aminoantipyrine(4-AAP)andsodium
N-ethyl-N-(3-sulfopropyl)m-anisidine(ESPA)toproduceaquinoneiminedyethatshowsanabsorbancemaximumat540nm.Theincreaseinabsorbanceat540nmisdirectlyproportionaltotriglycerideconcentrationofthesample.

Thetriglycerideandfreeglycerolkitsareforthequantitativedeterminationofglycerol,totaltriglyceridesorfreetriglycerides.

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Symbol 

  GHS07,GHS09