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Properties:
RelatedCategories | BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,InorganicIonsMetabolismAssayKits,Metabolomics |
Description:
PreparationNote
KitiscompatIBLewithserumandtissuesamplesbutnotplasmasamples.
Generaldescription
Ironisamineralthatplaysanessentialroleinmanybiologicalprocesses,includingirontransportandredoxreactions.Ironisatransitionelementthatcanformarangeofoxidationstates,themostcommonbeing
ironII(Fe2+orferrousiron)andironIII(Fe3+orferriciron).Iron-containingproteinsparticipateinmanyreactions,oftenutilizingtransitorychangesintheoxidationstateofirontocarryoutchemicalreactions.
Inthisassay,ironisreleasedbytheadditionofanacidicbuffer.SamplesmaybetesteddirectlytomeasureFe2+orreducedtomeasuretotaliron(Fe2+andFe3+).Releasedironisreactedwithachromagenresultinginacolorimetric(593nm)product,proportionaltotheironpresent.TheIronAssayKitprovidesasimpleconvenientmeansofmeasuringironinavarietyofbiologicalsamples.