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Properties:
RelatedCategories | BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,GlycolysisMetabolismAssayKits,Metabolomics |
Description:
Generaldescription
D-Lactate,typicallypresentinthebloodstreamatnanomolarconcentrations,isproducedbyanintestinalsourceorviathemethylglyoxalpathway.Inmammals,D-LactatemetabolismrequiresD-Lactatehydrogenaseandismetabolizedslowly,thusanincreaseinbloodconcentrationlevelscanleadtoacidemiaandacidosis.TheseverityofthisD-lacticacidosiscanbeassociatedwithneurotoxicsymptoms.SignificantD-Lactateaccumulationsinthebodycanalsoberelatedtoimpairedmetabolismandexcretion.
Inthisassay,D-LactateisspecificallyoxidizedbyD-Lactatehydrogenaseandgeneratesaproportionalcolorimetricproductmeasuredat450nm.Theusefulconcentrationrangeinsamplesis0.1-10mMD-Lactate.
SuitABIlity
Suitableforusewithsamplesofserum,plasma,cells,cultureandfermentationmedia.