Popular Documents: Bulletin(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,MetabolismAssayKitsA-Z,Metabolomics, |
| usage | sufficientfor100 colorimetric tests |
| storagetemp. | −20°C |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestions
areavailableforthisProduct.
Generaldescription
AscorbicAcid,alsoknownasVitaminC,isasix-carbonlactoneproducedbyplantsandsomeanimalspeciesbutnotbyhumansandotherprimates.Ascorbicacidfunctionsasanenzymaticcofactorformultipleenzymes,servingasanelectrondonorformonooxygenasesanddioxygenases.Ascorbicacidalsofunctionsasapowerfulantioxidant,particularlyinregardstoreactiveoxygenspecies.
Inthisassay,ascorbicacidconcentrationisdeterminedusingtheFerricReducing/AntioxidantandAscorbicAcid(FRASC)assay.Inthisassay,Fe3+isreducedtoFe2+byantioxidantspresentinthesample,whichresultsinacolorimetric(593nm)product.Theadditionofascorbateoxidasetoparallelsamplesoxidizesanyascorbicacidpresentallowingforthemeasurementoftheascorbicacidconcentration.
Thiskitissuitableforusewithcellandtissueculturesupernatantsaswellasserum,plasma,urine,andotherbiologicalfluids.
