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Sigma-Aldrich/Acid Phosphatase Activity Fluorometric Assay Kit/MAK087-1KT/1KT
ECNumber231-834-5
Popular Documents: Bulletin(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticActivityAssayKits,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,MetabolismAssayKitsA-Z, |
| usage | sufficientfor500 fluorometric tests |
| storagetemp. | −20°C |
| GeneInformation | human...ACP1(52),ACP2(53) mouse...ACP1(11431),ACP2(11432) rat...ACP1(24161),ACP2(24162) |
Description:
Generaldescription
Acidphosphatases(AP)areafamilyofenzymesthatnon-specificallycatalyzethehydrolysisofmonoestersandanhydridesofphosphoricacidtoproduceinorganicphosphateatanoptimalpHof4-7.ChangesinthelevelsandactivitiesofAPoccurinmanypatho-physiologicalconditionsincludingprostratecancerandbonedisease.
TheAPActivityFluorometricAssayKitprovidesasimpleanddirectprocedureformeasuringAPactivityinserumandothersamples.APactivityisdeterminedbytheconversionofthenon-fluorescent4-methyl-umbelliferylphosphatedisodiumsalt(MUP)tothefluorescentproduct4-methylumbelliferone(4-MU)(λex=360/λem=440nm),proportionaltotheAPactivitypresent.OneunitofAPistheamountofenzymethatwillhydrolyze1.0μmoleofMUPperminuteat25°C.
