Popular Documents: Bulletin(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | AssayKitsForStudyingFattyAcidMetabolism,BiochemicalsandReagents,BloodandUrineChemistryAssayKits,CancerMetabolism,CancerResearch, |
| usage | sufficientfor100 fluorometric tests |
| storagetemp. | −20°C |
Description:
Generaldescription
Thelipasefamilyofenzymescatalyzesthecleavageoftheesterbondsoflipids.Inmammals,thisfamilyincludesmanycriticalmembersincludingpancreatic,hepatic,endothelial,andlipoproteinlipase.Lipases,suchaspancreaticlipase,arecriticalforthemetabolismoflipids.Lipasesalsoplaykeyrolesinprocessessuchascellsignalingandinflammation.Measurementsoflipaseactivityarecommonlyusedtoscreenforpancreaticinjuryordisease,andtomonitordiseasessuchascysticfibrosis,celiacdisease,andCrohn′sdisease.
TheLipaseActivityAssayKitIIIprovidesasimpleanddirectprocedureformeasuringlipaseactivityinavarietyofsamples.Lipaseactivityisdeterminedusingacoupledenzymereaction,whichresultsinthegenerationofmethylresorufin(λex=529/λem=600nm)proportionaltotheenzymaticactivitypresent.Oneunitoflipaseistheamountofenzymethatwillgenerate1.0μmoleofmethylresorufinfromthesubstrateperminuteat37°C.
FeaturesandBenefits
CompatIBLewithhigh-throughputhandlingsystems.
