Popular Documents: Bulletin(PDF)
Properties:
| RelatedCategories | AssayKitsForStudyingFattyAcidMetabolism,BloodandUrineChemistryAssayKits,CancerMetabolism,CancerResearch,CellBIOLOGy, |
| storagetemp. | −20°C |
Description:
Generaldescription
Acetoacetate(AcAc)isaβ-ketoacidformedinthemitochondriabythecondensationoftwomoleculesofacetyl-CoA.AcAciseitherenzymaticallyreducedto3-hydroxybutyrateordecarboxylatedtoproduceacetone.Ketonebodies(AcAc,3-HB,andacetone)areusedasalternatesourceofenergyintheabsenceofglucose.ExcessketonebodiesareobservedintypeIdiabetes,alcoholism,orstarvation.
TheAcetoacetateColorimetricAssaykitprovidesasimpleandsensitiveprocedureformeasuringAcAcinvarioussamples(rangingfrom20-100nmole/well).AcAcconcentrationisdeterminedbygeneratingacolorimetric(550nm)productproportionaltotheamountofAcAcpresent.Thekitdoesnotdetect3-hydroxybutyrateandisspecificforacetoacetate.
SuitABIlity
Suitableforthedetectionofacetoacetateinsamplessuchasserum,plasma,blood,urine,andotherbodyfluids.
Safety:
GHS07
