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Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties:
Related Categories | Assay Kits For Studying Fatty Acid Metabolism, Blood and Urine Chemistry Assay Kits, Cancer Metabolism, Cancer Research, Carbohydrate Metabolism Assay Kits, |
usage | sufficient for 100 colorimetric tests |
storage temp. | −20°C |
Description:
General description
Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle. Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins. Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol.
The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples such as serum, tissue, cells, and food. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.