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Sigma-Aldrich/Pepsin from porcine gastric mucosa/P7000-25G/25G
CASNumber9001-75-6
ECNumber232-629-3
EnzymeCommission(EC)Number3.4.23.1 ( BRENDA
| IUBMB )MDLnumberMFCD00081840
eCl@ss42010127
Popular Documents: SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.4.x.xPeptidases,3.x.x.xHydrolases,ApplicationIndex,BiochemicalsandReagents,ClinicalChemistry, |
| form | powder |
| molwt | molwt35 kDa |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| shippedin | wetice |
| storagetemp. | 2-8°C |
| GeneInformation | pig...LOC396892(396892) |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestionsareavailableforthisProduct.
AnalysisNote
OptimumpHis2-4.Activein4Mureaand3MguanidineHCl.Stableat60 °C.PepsinisirreversIBLyinactivatedatpH8.0-8.5.
Application
PepsincleavagecanbeusedtoproduceF(ab′)2fragmentsofantibodies.pepsinatwww.Sigma-Aldrich.com/enz PepsinisapeptidaseusedtodigestproteinsandiscommonlyusedinthepreparationofFabfragmentsfromantibodies.Pepsin,fromporcinegastricmucosa,hasbeenusedtohydrolyzedrycervicalsamplesinmice.ProductP7000isprovidedasapowderandhasbeenusedtotreatepithelialcellsfromasinglefelinemammarycarcinoma. TheenzymefromSigmahasbeenusedtosimulateinvitrogastricdigestionofcookedcod.Ithasbeenusedtosimulateinvitrogastricdigestionofcocoamassandsupplementeddietaryfiber.Ithasalsobeenusedtoincreasethefractionofextractablesolublecollagenandtolowertheimmunogenicityoftheresultingcollagenfrombovinedermaltissue. Preferentialcleavage:hydrophobicandaromaticresiduesinP1andP1′postitions.CleavesPhe-Val,Gln-His,Glu-Ala,Ala-Leu,Leu-Tyr,Tyr-Leu,Gly-Phe,Phe-PheandPhe-Tyrbondsintheβchainofinsulin Pepsinhydrolyzespeptidebonds,notamideoresterlinkages.Pepsincleavespeptideswithanaromaticacidoneithersideofthepeptidebond.Sulfur-containingaminoacidsincreasesusceptibilitytohydrolysiswhentheyareclosetothepeptidebond.Pepsinpreferentiallycleavesatthecarboxylsideofphenylalanineandleucineandatthecarboxylsideofglutamicacidresidues.CleavesPhe-Val,Gln-His,Glu-Ala,Ala-Leu,Leu-Tyr,Tyr-Leu,Gly-Phe,Phe-PheandPhe-Tyrbondsintheβchainofinsulin Theenzymedoesnotcleaveatvaline,alanine,orglycinelinkages.Z-L-tyrosyl-L-phenylalani Viewmoreinformationonpepsinatwww.sigma-aldrich.com/enz 1kginpolybottle 25,100ginpolybottle OneunitwillproduceaΔA280of0.001perminatpH2.0at37 °C,measuredasTCA-solubleproductsusinghemoglobinassubstrate.(Finalvolume=16ml.Lightpath=1cm.)Biochem/physiolActions
Pepsinisthemajorproteolyticenzymeproducedinthestomach.Itdigestsproteinsthroughthecleavageofinteriorpeptidelinkages..OtherNotes
Packaging
UnitDefinition
Safety:
GHS07,GHS08
