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Sigma-Aldrich/Pepsin from porcine gastric mucosa/P7012-250MG/250MG
CASNumber9001-75-6
ECNumber232-629-3
EnzymeCommission(EC)Number3.4.23.1 ( BRENDA
| IUBMB )MDLnumberMFCD00081840
eCl@ss42010127
Popular Documents: ProductInformationSheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.4.x.xPeptidases,3.x.x.xHydrolases,Antibodies,AntibodyFragmentation,AntibodyModification, |
| QualityLevel | PREMIUM |
| material | off-whitetobeige |
| off-whitetoyellow | |
| form | lyophilizedpowder |
| molwt | molwt35 kDa |
| solubility | deionizedwater:soluble10 mg/mL |
| 10mMHCl:soluble4 mg/mL(Cold) |
Description:
AnalysisNote
E
OptimumpHis2-4.Activein4Mureaand3MguanidineHCl.Stableat60 °C.PepsinisirreversIBLyinactivatedatpH8.0-8.5.
ProteindeterminedbyE
Application
PepsincleavagecanbeusedtoproduceF(ab′)2fragmentsofantibodies.pepsinatwww.Sigma-Aldrich.com/enz PepsinisapeptidaseusedtodigestproteinsandiscommonlyusedinthepreparationofFabfragmentsfromantibodies.Pepsin,fromporcinegastricmucosa,hasbeenusedtohydrolyzedrycervicalsamplesinmice. TheenzymefromSigmahasbeenusedinthedigestionofcrudewheatgliADIn.Ithasbeenusedalongwithotherenzymestodemonstratetheeffectsoffixationandenzymaticdigestioninimmunohistochemicalassays,usingparaffinembeddedtissue.Ithasbeenusedfordigestion(beforeusingimmunoperoxidasetechniques)toreducenon-specificbackgroundstaininginsectionsofbronchialtissues.TheenzymehasalsobeenusedinthepreparationofF(ab)2fragmentfromIgG. Preferentialcleavage:hydrophobicandaromaticresiduesinP1andP1′postitions.CleavesPhe-Val,Gln-His,Glu-Ala,Ala-Leu,Leu-Tyr,Tyr-Leu,Gly-Phe,Phe-PheandPhe-Tyrbondsintheβchainofinsulin Pepsinhydrolyzespeptidebonds,notamideoresterlinkages.Pepsincleavespeptideswithanaromaticacidoneithersideofthepeptidebond.Sulfur-containingaminoacidsincreasesusceptibilitytohydrolysiswhentheyareclosetothepeptidebond.Pepsinpreferentiallycleavesatthecarboxylsideofphenylalanineandleucineandatthecarboxylsideofglutamicacidresidues.CleavesPhe-Val,Gln-His,Glu-Ala,Ala-Leu,Leu-Tyr,Tyr-Leu,Gly-Phe,Phe-PheandPhe-Tyrbondsintheβchainofinsulin Theenzymedoesnotcleaveatvaline,alanine,orglycinelinkages.Z-L-tyrosyl-L-phenylalani Viewmoreinformationonpepsinatwww.sigma-aldrich.com/enz 1,5,10,25,100ginpolybottle 250mginpolybottle OneunitwillproduceaΔA280of0.001perminatpH2.0at37°C,measuredasTCA-solubleproductsusinghemoglobinassubstrate.(Finalvolume=16mL.Lightpath=1cm.)Biochem/physiolActions
Pepsinisthemajorproteolyticenzymeproducedinthestomach.Itdigestsproteinsthroughthecleavageofinteriorpeptidelinkages.OtherNotes
Packaging
UnitDefinition
Safety:
GHS07,GHS08
