CASNumber9001-73-4
ECNumber232-627-2
EnzymeCommission(EC)Number3.4.22.2 ( BRENDA
| IUBMB )MDLnumberMFCD00131791
eCl@ss42030311
Popular Documents: Datasheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.4.x.xPeptidases,3.x.x.xHydrolases,Antibodies,AntibodyCharacterizationandAnalysis,AntibodyFragmentation, |
| form | lyophilizedpowder |
| molwt | molwt21 kDa |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| storagetemp. | −20°C |
Description:
Application
UsedtoproduceFabfragmentsofantibodies.Alsousedforcelldissociationsinceithasbeenshowntobemoreeffectiveandlessdamagingwithcertaintissues.
Papain,productP4762frompapayalatex,hasbeenusedtodigestcarotidarterysmoothmusclecells.Papainisusedindissectingsolutions.ItisusedtoproduceFabfragmentsofantibodies.Itisusedforcelldissociationsinceithasbeenshowntobemoreeffectiveandlessdamagingwithcertaintissues.
Biochem/physiolActions
Acysteineproteasethatcleavespeptidebondsofbasicaminoacids,leucine,orglycine.
pHoptimum6.0-7.0
Alsohydrolyzesestersandamides.
PapainpapayalatexhasantifungalactivityagainstC.albicans.Itisacysteineproteasethatcleavespeptidebondsofbasicaminoacids,leucine,orglycine.
.ThepHoptimumis6.0-7.0
.Papainhydrolyzesestersandamides.
OtherNotes
Viewmoreinformationonpapainatwww.Sigma-Aldrich.com/enz 1,5ginglassbottle 50,100,500mginglassbottle 25mginpolybottle 2×Crystallized LyophilizedpowdercontainingsodiumacetatePackaging
PreparationNote
Physicalform
Safety:
GHS07,GHS08
