CASNumber150308-80-8
LinearFormulaC₂₆H₂₅NO₅Se
MolecularWeight510.44 g/mol
Properties:
| RelatedCategories | AminoAcids&Derivatives,ChemicalBIOLOGy,ChemicalSynthesis,PeptideChemistry |
| brandfamily | NovABIochem |
| packaging | 1ginGlassbottle(8523460001) |
| 250mginGlassbottle(8523468250) | |
| shippedin | ambient |
| storagetemp. | Storeat+2°Cto+8°C. |
| specifications | Colour(visual):whitetoyellowtobeige |
| Appearanceofsubstance(visual):powder |
Description:
Backgroundinformation
BuildingblockfortheintroductionofselenocysteineduringFmocSPPS[1-3].Selenocysteinederivativescanundergoenantiomerizationduringcouplingandcanformdehydroalanineandβ-piperidinylalaninecontainingsideproductsduringsubsequentchainelongation[3].Therefore,activationmethods,suchasHBTUorPyBOPwhichinvolvetheadditionofatertiary,baseshouldbeavoidedforaddtionoftheSecandallsubsequentresidues.
Cleavageandside-chaindeprotectionofSec-containingpeptidescanbeeffectedusingtFA-m-cresol-thioanisole-EDT-water(80:5:5:5:5)[2]orTFA-water-DCM-TIS(89:5:51)at4°C[3].AstheSe-pMeOBzlbondisstabletoTFA,theseemthodsresultinformationofthecorrespondingSev(pMeOBzl)peptide.
PeptidescontainingtwoSec(pMeOBzl)residuescanbeoxidizeddirectlytothecorrespondingselenocystinylpeptidesbytreatmentwith5-10%DMSOinTFA[1-3].
Safety:
RelativelyunreactiveorganicreagentsshouldbecollectedincontainerA.Ifhalogenated,theyshouldbecollectedincontainerB.ForsolidresiduesusecontainerC.
