CASNumber9031-72-5
ECNumber232-870-4
EnzymeCommission(EC)Number1.1.1.1 ( BRENDA
| IUBMB )MDLnumberMFCD00081305
Popular Documents: Datasheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | 1.1.x.xActingonhydroxylgroups,1.x.x.xOxidoreductases,AlcoholMetabolism,ApplicationIndex,BiochemicalsandReagents, |
| QualityLevel | PREMIUM |
| form | lyophilizedpowder(containsbuffersalts) |
| molwt | Mw141-151 kDa |
| purifiedby | crystallization |
| greeneralternativeproductcharacteristics | WastePrevention:Greeneralternativeproductcharacteristics LearnmoreaboutthePrinciplesofGreenChemistry. |
| solubility | H2O:soluble1.0 mg/mLcleartoslightlyhazy,colorlesstofaintlyyellow |
| :soluble |
Description:
Caution
Containsboundβ-NADandβ-NADHandisnotsuitablefortherecyclingmicroassayofβ-NADandβ-NADH.IfyourequireADHforthispurpose,seeCatalogNo.A3263.
Generaldescription
SigmaLifeScienceiscommittedtobringingyou Greener AlternativeProducts,whichadheretooneormoreofThe12Principlesof Greener Chemistry.Thisproducthasbeenenhancedforwastepreventionwhenusedinfuelcellresearch.Formoreinformationseethearticleinbiofiles.
Packaging
7500,15000,30000,75000,150000,300000unitsinpolybottle
PreparationNote
Dissolvesinwaterataconcentrationof1mg/mLtoformacleartoslightlyhazy,colorlesstofaintlyyellowcoloredsolution.
UnitDefinition
Oneunitwillconvert1.0 μmoleofethanoltoacetaldehydeperminatpH 8.8at25 °C.
Physicalform
Solidscontaining≤2%citratebuffersalts
Application
Alcoholdehydrogenasehasbeenusedalongwithlacticdehydrogenasefortheenzymaticreductionofacetaldehydeusingsodium(R,S)-[2-3H]lactate.EthanolconcentrationcanbedeterminedcolorimentricallybymonitoringtheenzymaticreductionofNADusingalcoholdehydrogenaseafterpreremovalofthealdehydegroup.
Biochem/physiolActions
ADH(alcoholdehydrogenase)isoneofthefirstenzymestobeisolatedandpurified.NAD+isitscoenzyme.ThreeisozymesofyeastADH,thatis,yeastalcoholdehydrogenase-1,2and3(YADH-1,-2,-3)havebeenidentified.YADH-1isexpressedduringanaerobicfermentation,YADH-2isexpressedinthecytoplasmandYADH-3islocalizedtothemitochondria.A141kDatetramercontaining4equalsubunits.Theactivesiteofeachsubunitcontainsazincatom.Eachactivesitealsocontains2reactivesulfhydrylgroupsandahistidineresidue.
Isoelectricpoint:5.4-5.8
OptimalpH:8.6-9.0
Substrates:YeastADHismostactivewithethanolanditsactivitydecreasesasthesizeofthealcoholincreasesordecreases.Branchedchainalcoholsandsecondaryalcoholsalsohaveverylowactivity.
KM(ethanol)=2.1×10-2M
KM(methanol=1.3×10-1M
KM(isopropanol)=1.4×10-1M
Inhibitors:Compoundsthatreactwithfreesulfhydryls,includingN-alkylmaleimidesandiodoacetamide.
Zincchelatorinhibitors,including1,10-phenanthroline,
8-hydroxyquinoline,2,2′-dipyridyl,andthiourea.
Substrateanalogueinhibitors,includingβ-NADanalogs,purineandpyrimidinederivatives,chloroethanol,andfluoroethanol.
ExtinctionCoefficient:E1%=14.6(water,280nm)
