Popular Documents: Bulletin(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | AerobicGlycolysis(theWarburgEffect),AerobicGlycolysisMetabolitesandEnzymes,AssayKitsforStudyingAerobicGlycolysisMetabolitesandMetabolicEnzymes,BloodandUrineChemistryAssayKits,CancerMetabolism, |
| usage | sufficientfor500 colorimetric tests |
| storagetemp. | −20°C |
| GeneInformation | human...LDHA(3939),LDHB(3945) mouse...LDHA(16828),LDHB(16832) rat...LDHA(24533),LDHB(24534) |
Description:
Generaldescription
Lactatedehydrogenase(LDH)isanoxidoreductaseenzymethatcatalysestheinterconversionofpyruvateandlactate.CellsreleaseLDHintothebloodstreamaftertissuedamageorredbloodcellhemolysis.SinceLDHisafairlystableenzyme,ithasbeenwidelyusedtoevaluatethepresenceofdamageandtoxicityoftissueandcells.LDHisalsoelevatedincertainpathologicalconditionssuchascancer.QuantificationofLDHhasabroadrangeofapplications.
Inthiskit,LDHreducesNADtoNADH,whichisspecificallydetectedbycolorimetric(450nm)assay.TheLDHActivityAssaykitquantifiesLDHactivityinvarietyofbiologicalsamples.Theassayisquick,convenient,andsensitive.
Application
LactateDehydrogenaseActivityAssayKithasbeenusedformeasuringtheactivityoflactatedehydrogenase(LDH)inthecellculturemedium.
SuitABIlity
SuitableforthedetectionofL(+)-Lactateinbiologicalsamplessuchasserum,plasma,cells,cultureandfermentationmedia.
