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Properties:
RelatedCategories | AerobicGlycolysis(theWarburgEffect),AerobicGlycolysisMetabolitesandEnzymes,AssayKitsforStudyingAerobicGlycolysisMetabolitesandMetabolicEnzymes,BloodandUrineChemistryAssayKits,CancerMetabolism, |
usage | sufficientfor100 colorimetric tests |
storagetemp. | −20°C |
Description:
Generaldescription
L(+)-LactateisametaboliccompoundformedinanimalsbytheactionoftheenzymeLactateDehydrogenase.Lactateisproducedinproliferatingcellsandduringanaerobicconditionssuchasexercise.Abnormallyhighconcentrationsoflactatehavebeenrelatedtopathologicalconditionssuchascancer,diabetes,andlactateacidosis.L(+)-Lactateisthemajorstereoisomeroflactateformedinhumanintermediarymetabolismandispresentinbloodatlevelsofaround1-2mmol/L.ThiskitprovidesaconvenientmeansfordetectingL(+)-Lactateinbiologicalsamples.
Lactateconcentrationisdeterminedbyanenzymeassay,whichresultsinacolorimetric(450nm)product,proportionaltthelactatepresent.Thereisnoneedofpretreatmentorpurificationofsamples.Typicalsensitivitiesofdetectionarebetween0.02-10mMoflactate.
SuitABIlity
SuitablefordetectingL(+)-Lactateinculturemedium,fermentationmedium,bloodandcells.