CASNumber9007-34-5
ECNumber232-697-4
MDLnumberMFCD00130825
Popular Documents: ProductInformationSheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | ApplicationIndex,AttachmentFactors,BiochemicalsandReagents,CellCulture,ClinicalChemistry, |
| description | SigmaTypeVI |
| form | powder |
| impurities | HIV,hepatitisBandhepatitisC,nonedetected |
| solubility | 0.5Maceticacid:1 mg/mL |
| suitABIlity | suitableforsubstrateforCollagenase |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| storagetemp. | −20°C |
Description:
Components
Allcollagenmoleculesarecomposedofthreepolypeptidechainsarrangedinatriplehelicalconformation,withaprimarystructurethatismostlyarepeatingmotifwithglycineineverythirdpositionandprolineor4-hydroxyprolinefrequentlyprecedingtheglycineresidue.TypeIVcollagenoccursonlyinthebasementmembranesandcontainsuptosixgeneticallydistincta-chains.
AnalysisNote
AnSDSpolyacrylamidegelelectrophoresistestrununderreducingconditionsconsistentwithbasementmembranecollagenyieldsthreemajorbands.
OtherNotes
Collagenisclassifiedintoanumberofstructurallyandgeneticallydistincttypes.WeusethenomenclatureproposedbyBornsteinandTraub.BewaryofconfusingSigma-typedesignationswithrecognizedcollagenclassificationtypes.
Packaging
10,50mginpolybottle
5mginglassbottle
PreparationNote
Thisisalyophilizedpowderthatcanbereconstitutedinsterile.5Maceticacid,PBSorwaterat1mg/mL.APBSsolutionwillbestableforatleastoneyearat-20°C.ItispreparedbyamodificationofthepepsinextractionmethodofGlanville,R.W.,etal.
Biochem/physiolActions
Duringdevelopment,collagenIVisubiquitouslydistributedinBMs.Duringthematurationprocess,thisnetworkgetspartiallyreplacedinaremarkablytissuespecificmanner,definingBMstructureandfunction.CollagenIVhasbeenshowntobindtoplatelets,hepatocytes,keratinocytes,endothelial,mesangial,pancreaticcellsandsometumorcells.
