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Sigma-Aldrich/Acetylcholinesterase from Electrophorus electricus (electric eel)/C3389-500UN/500UN
CASNumber9000-81-1
ECNumber232-559-3
EnzymeCommission(EC)Number3.1.1.7 ( BRENDA
| IUBMB )MDLnumberMFCD00081268
Popular Documents: ProductInformationSheet(PDF) | SpecificationSheet(PDF)
Properties:
| RelatedCategories | 3.1.x.xActingonesters,3.x.x.xHydrolases,Acetylcholinesterase(AChE),True,RBCorErythrocyteCholinesterase,E.C.3.1.1.7,AnalyticalandIndustrialEnzymes,ApplicationIndex, |
| type | TypeVI-S |
| form | lyophilizedpowder |
| composition | Protein,≥60%biuret |
| solubility | 20mMTrisHClbuffer,pH7.5:soluble1.0 mg/mLclear(lit.) |
| FeaturedIndustry | DiagnosticAssayManufacturing |
| storagetemp. | −20°C |
Description:
AnalysisNote
Theactivityobtainedusingacetylcholineassubstrateis30-100timesthatobtainedwithbutyrylcholine,usingacetylcholinesterasefromelectriceel.
Biochem/physiolActions
Majordegradativeenzymeforacetylcholineinvivo.Convertsacetylcholine+H2Otocholine+aceticacid.
Packaging
10000unitsinpolybottle
500,2000unitsinglassbottle
PreparationNote
Thisenzymedissolvesin20mMTris-HClbuffer,pH7.5at1mg/mLconcentration,yieldingaclearsolution.
UnitDefinition
Oneunitwillhydrolyze1.0μmoleofacetylcholinetocholineandacetateperminatpH8.0at37°C.
Physicalform
LyophilizedpowdercontainingTrisbuffersalts
Application
Theenzymefromsigmahasbeenusedasareferencetotoevaluatetheeffectofaspartamemetabolitesonhippocampalacetylcholinesteraseactivity.Theenzymehasalsobeenusedinimmobilizationstudiesfortherapiddetectionofacetylthiocholinechloride.
Generaldescription
MolecularWeight:280kDa
IsoelectricPoint:5.5
ExtinctionCoefficient:E1%=18.0(280nm)
AcetylcholinesterasefromElectrophoruselectricusisatetramercomposedof4equalsubunitsof70kDaeach.Eachsubunitcontainsoneactivesite.Theenzymeisaglycoproteincontaininghexosamines.
