
EnzymeCommission(EC)Number3.2.1.1 ( BRENDA
| IUBMB
)
MDLnumberMFCD00081319
eCl@ss32160410
Popular Documents: ProductInformationSheet(PDF) | SpecificationSheet(PDF)
Properties:
RelatedCategories | α-Amylase,3.2.x.xGlycosidases,3.x.x.xHydrolases,Amylases,α-Glucosidase,Starches(AmyloseandAmylopectin)andGlycogen,ApplicationIndex, |
QualityLevel | PREMIUM |
type | TypeI-A |
form | salinesUSPension |
greeneralternativeproductcharacteristics | WastePrevention:Greeneralternativeproductcharacteristics LearnmoreaboutthePrinciplesofGreenChemistry. |
FeaturedIndustry | DiagnosticAssayManufacturing |
greeneralternativecategory | Enabling |
storagetemp. | 2-8°C |
Description:
Generaldescription
SigmaLifeScienceiscommittedtobringingyou Greener AlternativeProducts,whichadheretooneormoreofThe12Principlesof Greener Chemistry.Thisproducthasbeenenhancedforwastepreventionwhenusedinstarchethanolresearch.Formoreinformationseethearticleinbiofiles.
Molecularmass:51-54kDa.
α-Amylaseisolatedfromporcinepancreasisaglycoprotein.Itisasinglepolypeptidechainof~475residuescontainingtwoSHgroupsandfourdisulfidebridgesandatightlyboundCa2+necessaryforstABIlity.Chlorideionsarenecessaryforactivityandstability.ThepHrangeforactivityis5.5to8.0,withthepHoptimumat7.
OtherNotes
Viewmoreinformationonenzymesforcomplexcarbohydrateanalysisatwww.Sigma-Aldrich.com/enz 2×crystallized Oneunitwillliberate1.0mgofmaltosefromstarchin3minatpH6.9at20°C. Suspensionin2.9MNaClsolutioncontaining3mMCaCl2. α-Amylaseisusedtohydrolyzeαbondsofα-linkedpolysaccharides,suchasstarchandglycogen.α-Amylase,fromSigma,hasbeenusedinvariousplantstudies,suchasmetabolismstudiesinArabidopsis. α-Amylasehydrolyzestheα-(1,4)glucanlinkagesinpolysaccharidesofthreeormoreα-(1,4)linkedD-glucoseunits.Naturalsubstratessuchasstarchandglycogenarebrokendownintoglucoseandmaltose.α-Amylase,fromporcinepancreas,isaglycoproteinthatconsistsofasinglepolypeptidechainofapproximately475residuescontaining2SHgroupsandfourdisulfidebridgesandatightlyboundCa2+necessaryforstability.PreparationNote
UnitDefinition
Physicalform
Application
Biochem/physiolActions
Safety:
GHS08