eCl@ss32110501
Properties:
| RelatedCategories | 3μmTSKgelNH2-100Columns,Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHILICHPLCColumns, |
| material | stainlesssteelhardware |
| mfr.no. | Tosoh,21970 |
| parameter | 0-100%organicsolvent |
| 1.0mL/minstandardflowrate | |
| 10-50°Ctemperature | |
| 150barmax.pressure | |
| application(s) | HPLC:suitable |
Description:
Physicalform
Shippedin85%acetonitrile-15%water.
Generaldescription
TSKgelNH2-100aminocolumnsexpandtherangeofTSKgelcolumnsforhydrophilicinteractionchromatography(HILIC).OfferingadifferentselectivityfromthewellknownTSKgelAmide-80columns,thesenovelamino-bondedphasecolumnsstandoutbyprovidingmuchimprovedchemicalstability,aprerequisiteforachievingreproducIBLeandreliableresults.TSKgelNH2-100columnsarepackedwith3micronsilicaparticlescontaining100Åpores.Anovelbondingstrategywasadoptedtoimprovechemicalstabilityofthebondedphase.Firstthesilicaisreactedwithatrimethylsilaneendcappingreagentatalowstoichiometricratio,beforereactingresidualandaccessiblesilanolgroupswithtrifunctionalalkylaminosilanereagent.Theresultingbondedphaseprovidesabettersafeguardagainsthydrolysisoftheunderlyingsilica.TSKgelNH2-100columnsareuniqueinthatthebondedphaseligandnotonly,asexpected,hasaterminalprimaryaminogroup,butthatthespaceralsoincorporatessecondaryaswellastertiaryaminogroups.Anioniccompoundsareretainedonthecolumnbyionicinteraction.ThisallowsfortheuseofgrADIentsinsaltconcentration,inadditiontogradientelutionswithacetonitrile.SincetheTSKgelNH2-100columnshavecationicsites,thecolumnscanbeusedasmixedmodecolumnsundersomeconditions.TSKgelNH2-100columnsarewellsuitedfortheanalysisofalltypesofhydrophiliccompounds,includingcarbohydratesandpeptides.Duetoahighliganddensityandlargesurfacearea,thesecolumnsshowstrongerretentionofpolarcompoundsthanTSKgelAmide-80columns.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation
