eCl@ss32110501
Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,TSKgelPhenyl-5PWRPColumns(1000Åpores), |
| material | stainlesssteelhardware |
| mfr.no. | TosohPhenyl-5PW,16260 |
| extentoflabeling | 1 meq/mLliganddensity(estimate) |
| parameter | saltconcentration |
| 0-100%organicsolvent | |
| 30barmax.pressure | |
| 4-60°Ctemperature |
Description:
Generaldescription
Polymer-basedparticlesforreversed-phase(RP)HPLCgenerallyhavetwobenefitsoversilica-basedparticles:TheyaremoretolerantofhighandlowpHoperatingconditionsandtheydonothavethesilanolinteractionsthatcausepeaktailingofsomebasicanalytes.Also,somesilicashaveanintrinsicmetalcontentthatmaycausechelationproblemswithsensitivebiomolecules,leADIngtopoorrecovery.PolymericTSKgelcolumnscomeinC18andphenylfunctionalgroupsandarangeofparticleporosities.Fastkineticsandquantitativeproteinrecoveryatsubmicrogramloadingarecharacteristicofnonporousresin-basedTSKgelOctadecyl-NPRcolumns.
• Octadecyl-2PWparticleshave125Åpores
• Octadecyl-4PWparticleshave500Åpores
• Phenyl-5PWRPparticleshave1000Åpores
• Octadecyl-NPRparticlesarenonporous
TSKgelPhenyl-5PWRPofferssimiliarselectivitytoitshydrophobicinterationcounterpartTSKgelPhenyl-5PW,althoughahigherdegreeofcross-linkingallowsforincreasedstABIlityinaqueous/organicmobilephasegradients.Thehighlycross-linkedpolymethacrylatebasematerialprovidesanadvantageoversilicawhenhighpHbuffersystemsareneeded.Additionally,TSKgelPhenyl-5PWRPcanwithstandrigorouscleaningprotocolsusingeitheracidorbase.The1000Åporesizeaccommodatesglobularproteinsamplesupto1,000,000Da.
Physicalform
Shippedin30%methanol-70%water.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation
