eCl@ss32110501
Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,HydrophobicInteraction(HIC)Columns,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories, |
| material | stainlesssteelhardware |
| mfr.no. | TosohPhenyl-5PW,18759 |
| parameter | ≤4Msaltconcentration |
| ≤50%organicsolvent | |
| 0.12mL/minflowrate | |
| 10-50°Ctemperature | |
| 8barmax.pressure |
Description:
Generaldescription
Bothhydrophobicinteractionchromatography(HIC)andreversed-phaseliquidchromatographyseparateonthebasisofproteinhydrophobicityandallowselectivebindinganddesorptionofproteins.However,HICoperatesatsignificantlylowerbindingenergyandusesaqueousmobilephases.Thesecharacteristicsprovideagentletechniquethatislesslikelytodisturbproteinconformation.Asaresult,HICgenerallyprovidesbetteractivityrecovery.
TSKgelBioAssistPhenylcolumnscontainthesameparticlesasPhenyl-5PWcolumns,butarepackedinPEEKcolumnhardwareratherthanstainlesssteel.BioAsistPhenyl(Phenyl-5PW),TSKgelEther-5PWandButyl-NPRresin-basedcolumnsprovidearangeofhydrophobicitiesforchromatographicoptimization.Ammoniumsulfateconcentrationcanbeminimizedbyusingamorehydrophobicpacking,suchasBioAssistPhenyl.WerecommendanEther-5PWcolumnforpurifyingveryhydrophobicproteins.TSKgelBioAssistPhenylandTSKgelEther-5PWpackingsarebasedonTSKgelG5000PWresin-10Â μmparticleswith1000Â Ã…pores.TSKgelButyl-NPRcolumns,whicharelesshydrophobicthanEther-5PW,ispreparedfrom2.5Â μmnonporousparticles,allowingforrapidanalysesandsemi-micropreparativeapplications.Allthreecolumntypescanbecleanedwith0.2Â MNaOH.Scale-upcanbeperformedbyusingcorrespondingToyopearlbulkresins.
ThemosthydrophobicamongthethreeTSKgelHICcolumns,TSKgelPhenyl-5PWisanexcellentchoicetoscreenfortheselectivity,retentionandrecoveryofmostbiomolecules.Featuringa1000Åporesizeinapolymethacrylatebasematerial,thePhenyl-5PWcolumnisstableineitheracidorcausticcleaningregimensandprovidesexcellentaccesstolargermoleculeswithlowdiffusioncoefficients.StainlesssteelisthestandardcolumnformatforTSKgelPhenyl-5PW.GlassandPEEKcolumnhousingsareavailableforsamplesthatmayadsorbtostainlesssteel.Thealternativecolumnformatsarealsosuitableforgeneralapplications.
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LegalInformation
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