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Sigma-Aldrich/TSKgel® BioAssist® Chelate HPLC Column/820022/
eCl@ss32110501
Properties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelAffinityHPLCColumns,TSKgelBioAssistPEEKHPLCColumns, |
material | PEEKhardware |
mfr.no. | Tosoh,20022 |
extentoflabeling | 20 µmol/mLresinliganddensity |
parameter | ≤20%organicsolvent |
≤3Msaltconcentration | |
≤45°Ctemperature | |
1.2mL/minflowrate |
Description:
Generaldescription
Highefficiency,resin-basedTSKgelaffinitycolumns(10μmparticles)separateorpurifymanyenzymesandotherproteins.
TSKgelChelate-5PWcolumnsutilizethetendencyofiminodiaceticacid(IDA)toformacomplexwithmetalions,suchasZn2+,Ni2+andCu2+.Thecolumnisloadedwithdivalentmetalionsbychelation.PeptidesandproteinscontaininghistidineresidueswillnormallyadsorbtothesechelatedionsatneutralpH.Theretainedcompoundscanbeelutedwithbuffercontainingimidazoleorglycine.Thekeytomakingsuccessfuluseofthisretentionmechanismistheproperselectionofmetalionsforchelationandtheelutionbuffertodesorbtheanalytes.Ingeneral,Cu2+,interactsbetterwithproteins;however,resolutionisusuallyenhancedwithZn2+.AgrADIentmobilephasecontainingincreasingimidazoleorglycineconcentrationsisusedtoelutetheretainedcompounds.Alternatively,adecreasingpHgradientcanbeusedforelutionpurposesaswell.
Applicationsinclude:immunoglobulins,transferrin,lectins,milkproteins,membraneproteinsandpeptides.
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LegalInformation
BioAssistisaregisteredtrademarkofTosohCorporation
TSKgelisaregisteredtrademarkofTosohCorporation