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Sigma-Aldrich/ToyoScreen® Butyl-650M Process Development Column/821377/
Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,ToyoScreenHydrophobicInteractionProcessDevelopmentColumns, |
| material | aluminumseal |
| polypropyleneandpolyethylenefrit | |
| packaging | pkgof6×5 mL |
| mfr.no. | Tosoh,21377 |
| parameter | ≤50%organicsolvent |
| 1-5mL/minflowrate | |
| 20mL/minmax.flowrate |
Description:
Generaldescription
Everylargescalecommercialpurificationbeginsatasmallscaleinamethoddevelopmentlabbyevaluatingretentionandselectivityofthetargetproteinversusimpurities,hostcellproteins,DNA,etc.,onmultipleresins,oftenfromdifferentvendors.Dependingontheconstraints,theprocessdevelopmentengineercaneitherpurchasebulkresinstopackseveralsmallcolumnswithdifferentresinsortakeadvantageofthespeedandconvenienceofpre-packedcolumnsforresinevaluation.Pre-packedToyoScreen®ProcessDevelopmentColumnsareavailablein1mLand5mLvolumespackedwiththemostpopularToyopearl®resins.ToyoScreencolumnscanbeusedtoquicklyestablishoptimumelutionconditions.VaryingpH,salttype,grADIentconditionsandflowratearecommonexperimentalparametersexplored.InitialresultsfromresinscreeningandoptimizationwithToyoScreencolumnsaccuratelypredicttheseparationbehavioratlargerscale.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
Specifications
Cleaninplacewith0.5MNaOHor0.1MHCl.
LegalInformation
ToyoScreenisaregisteredtrademarkofTosohCorporation
ToyopearlisaregisteredtrademarkofTosohCorporation
