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Sigma-Aldrich/ToyoScreen® AF-rProtein A HC-650F Process Development Column/823431/
Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,ToyoScreenAffinityProcessDevelopmentColumns, |
| material | aluminumseal |
| polypropyleneandpolyethylenefrit | |
| packaging | pkgof1×5 mL |
| mfr.no. | Tosoh,23431 |
| parameter | 1-5mL/minflowrate |
| 20mL/minmax.flowrate | |
| 3barmax.pressure |
Description:
Generaldescription
Everylargescalecommercialpurificationbeginsatasmallscaleinamethoddevelopmentlabbyevaluatingretentionandselectivityofthetargetproteinversusimpurities,hostcellproteins,DNA,etc.,onmultipleresins,oftenfromdifferentvendors.Dependingontheconstraints,theprocessdevelopmentengineercaneitherpurchasebulkresinstopackseveralsmallcolumnswithdifferentresinsortakeadvantageofthespeedandconvenienceofpre-packedcolumnsforresinevaluation.Pre-packedToyoScreen®ProcessDevelopmentColumnsareavailablein1mLand5mLvolumespackedwiththemostpopularToyopearl®resins.ToyoScreencolumnscanbeusedtoquicklyestablishoptimumelutionconditions.VaryingpH,salttype,grADIentconditionsandflowratearecommonexperimentalparametersexplored.InitialresultsfromresinscreeningandoptimizationwithToyoScreencolumnsaccuratelypredicttheseparationbehavioratlargerscale.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
Specifications
Cleaninplacewith0.1MNaOH.
LegalInformation
ToyoScreenisaregisteredtrademarkofTosohCorporation
ToyopearlisaregisteredtrademarkofTosohCorporation
