Properties:
| Related Categories | Analytical/Chromatography, GE Life Sciences Gel Filtration Columns, Gel Filtration Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, |
| material | Bed Support (polyamide) |
| borosilicate glass column | |
| glass fiber (reinforced) | |
| shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| mol wt | mol wt 3000-70000 Da (globular proteins) |
| packaging | pkg of 1 ea |
| mfr. no. | GE Healthcare, 28-9893-33 |
Description:
Analysis Note
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com
.
Features and Benefits
• High resolution with short run times and good recovery.
• High capacity at high flow rates.
• Separations can be scaled up to production levels.
• Prepacked for convenience and reproducibility.
• 1/16” fittings for convenient connections to AKTA design systems.
General description
HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
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Suitability
All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides
Legal Information
HiLoad is a registered trademark of GE Healthcare
Superdex is a registered trademark of GE Healthcare
