Properties:
| Related Categories | Analytical/Chromatography, GE Life Sciences Gel Filtration Columns, Gel Filtration Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, |
| material | Bed Support (polyamide) |
| borosilicate glass column | |
| polypropylene fiber (reinforced glass) | |
| shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| mol wt | mol wt 3000-70000 Da (globular proteins) |
| packaging | pkg of 1 ea |
| mfr. no. | GE Healthcare, 17-5174-01 |
Description:
Analysis Note
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com
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Features and Benefits
• High-resolution separations of proteins, peptides, and other biomolecules according to size.
• Well suited for the polishing step in a purification procedure.
• Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
• Easy and predictable scale-up.
• Excellent reproducibility and durability.
• For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns.
General description
Superdex® 75 10/300 GL is a prepacked gel filtration column for high-resolution, semipreparative, and analytical separations of biomolecules. Superdex® 75 has a separation range for molecules with molecular weights between 3 000 and 70 000.
Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family excellent reproducibility and durability in purification using a chromatography system.
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Suitability
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
for high-resolution, semipreperative and analytical separations of biomolecules
Legal Information
Superdex is a registered trademark of GE Healthcare
