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Sigma-Aldrich/HiPrep™ 16/60 Sephacryl® S-400 HR/GE28-9356-04/
Properties:
Related Categories | Analytical/Chromatography, GE Life Sciences Gel Filtration Columns, Gel Filtration Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, |
material | polypropylene (PP) |
shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
mol wt | mol wt 20000-8000000 Da (globular proteins) |
packaging | pkg of 1 ea |
mfr. no. | GE Healthcare, 28-9356-04 |
parameter | <0.5 mL/min flow rate |
0.15 Mpa (22 psi) |
Description:
Analysis Note
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.
Application
Sephacryl® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.
Features and Benefits
• Gel filtration columns for low to medium pressure AKTA systems.
• The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
General description
HiPrep™ 16/60 Sephacryl® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.
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Suitability
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14
Legal Information
HiPrep is a trademark of GE Healthcare
Sephacryl is a registered trademark of GE Healthcare