Properties:
| RelatedCategories | Analytical/Chromatography,GEHealthcareLifeSciencesProteinSamplePreparationFormats,HiTrap&PD-10Cartridges,HiTrapSystem/syringe,MolecularBIOLOGy, |
| shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
| packaging | packof5×1 mL |
| mfr.no. | GEHealthcare,17-0920-03 |
| parameter | <4mL/minflowrate(Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| 42psi | |
| bedsize | 7 mm×25 mm |
| bedvolume | 1 mL |
Description:
AnalysisNote
www.geLifesciences.com
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LegalInformation
IMACSepharoseproducts,NiSepharoseproductsandFeSepharoseproducts
TheseproductsaresoldunderalicensefromSigma-AldrichunderpatentnumberEP1276716(Metalchelatingcompositions)andequivalentpatentsandpatentapplicationsinothercountries.
HiTrapisaregisteredtrademarkofGEHealthcare
SepharoseisaregisteredtrademarkofGEHealthcare
Application
IMACSepharose®HPissuppliedfreeofmetalions.Itischargedbytheuserwiththetransitionmetalionofchoice(e.g.Cu2+,Zn2+,Ni2+,orCo2+);thesemetalionswillbindtothecovalentlyimmobilizedchelatingligandontheSepharose.
FeaturesandBenefits
• Foroptimizingpurificationofhistidine-taggedProteinswhenNi2+isnotthebestchoiceofmetalion.
• Convenientlychargewithyourmetalofchoice.
• Smallbeadsizeforhigh-performancepurification.
• Highbindingcapacity.
Generaldescription
HiTrap®IMACHPisprepackedwithIMACSepharose®HighPerformance.ThemediumischargedwiththemetalionofyourchoiceforImmobilizedMetalionAffinityChromatography(IMAC)andsubsequenthigh-resolutionpurificationofpolyhistidinetaggedproteins.
Safety:
GHS02
