eCl@ss32110501
Properties:
| RelatedCategories | 3micronDiscoveryBIOWidePore(300)C5Columns,Analytical/Chromatography,C5forBiopolymers,CapillaryDimensions,CapillaryandMicroboreDimensions, |
| feature | endcapped |
| extentoflabeling | 3.5%CarbonloADIng |
| application(s) | HPLC:suitable |
| L×I.D. | 10 cm×1 mm |
| surfacecoverage | 4.5 μmol/m2 |
| matrix | silicaparticleplatform |
| matrixactivegroup | C5(pentyl)phase |
Description:
Generaldescription
DiscoveryBIOWidePoreC5wasdesignedfortheefficientandreliableseparationofproteinsandpeptides,especiallyhydrophobicpeptides,byRP-HPLC.Long-chainphases,likeC8orC18,areoftentoohydrophobicforproteinsandcancauseexcessivelylongretentiontimeorevenirreversIBLebindingtothecolumn.Forthisreasonshort-chainphases,typicallyC3orC4,areoftenusedforRP-HPLCofproteins.However,theseshort-chainphasesaresusceptibletohydrolysis,resultinginshortcolumnlifetime,especiallyatlowpH.TheDiscoveryBIOWidePoreC5giveselutionordersimilartoaconventionalC4,yethasenhancedpHstABIlityforlongercolumnlifetime.Generally,higherefficiencyseparationsareachievableontheDiscoveryBIOWidePoreC5becauseoftheimprovementswehavemadetothesilicaandbonded-phasechemistry.
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LegalInformation
DiscoveryisaregisteredtrademarkofSigma-AldrichCo.LLC
